Accumulation of Taxadiene by Root Culture of Nicotiana Benthamiana Domin Transformed with Taxadiene Synthase
نویسندگان
چکیده
Nicotiana benthamiana was transformed with a taxadiene synthase (TS) gene for the de novo synthesis of taxadiene. The successfully transformed lines of N. benthamiana producing taxadiene was further cultivated for root culture to produce taxadiene in in vitro system. The roots of lines TSS-5, TSS-7, TSS-8 and TSS-10 accumulated 14.6 22.5 μg/g (dw) taxadiene. Methyl jasmonate treatment to the TSS-8 line for 7 or 14 days increased the accumulation of taxadiene by 1.17 and 1.88-folds, respectively. Data suggested that the root culture of TS gene transformed N. benthamiana is an ideal system for the stable and efficient production of taxadiene.
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